The Use of Different Hormone Concentrations During the Propagation of Tea Plant by Tissue Culture
Abstract:
Propagation of tea plants in vitro provides a fast technology for producing a large number of genetically superior and pathogen-free plant materials in a limited time and space. However, in comparison with ex vitro propagation methods, propagtion of plants in vitro reduces the acclimatization rate. The low survival rate of micropropagated plantlets after transfer to natural ex vitro condition has made the use of these propagation techniques economically unviable for many species (Nguyen et al., 1999). Tea is one of the plants that is very difficult to reproduce in tissue culture. For this reason, the fact that the plant faces some difficulties in plant sterilization at the time of propagation by tissue culture causes this plant to be placed in the order of plants that are difficult to propagate for tissue culture. The tea plant is a very important plant variety for our country, and traditional varieties are faced with the fear of extinction, making it necessary to try different conditions to increase this plant variety. One of these ways required the propagation of our local variety, which grows wild and civilized in nature in the province of Lankaran, by tissue culture. Although sterilization of the tea plant in tissue culture is a bit difficult, we made the plant materials sterile by using liquid soap, alcohol and mercury chloride in the laboratory environment and planted them in the nutrient medium in a laminar cabinet in the Modified MS environment. Different concentrations of BAP, IBA, IAA, NAA, GA3 hormones were tested in the nutrient medium during the growing period of the plant. In the rooting part, different concentrations of IBA, IAA, NAA hormones were tried and the most suitable and the most appropriate dose was determined.
