The Oral Carriage of Candida and Risk Habits of a Cohort of Sri Lankan Male Patients with Oral Fibroepithelial Polyps
Abstract:
There is an emerging threat on multi drug resistant Candida species in the time of global elderly population is increas ing. The oral cavity is the main reservoir for Candida species. The aim of this study was to discover oral carriage and oral risk habits of Candida in a cohort of Sri Lankan male patients with oral fibroepithelial polyps. DNA was extracted from 27 intra-oral fibro-epithelial polyps [FEP]) and sequenced for the fungal internal transcribed spacer 2 region using Illumina™ 2 x300bp chemistry. Merged reads were classified to species level using a BLASTN-algorithm with UNITE’s named species sequences as reference. Fibroepithelial polyps are inflammatory hyperplastic lesions charac terized by para-keratinized stratified squamous epithelium. They develop in response to chronic irritation and display arcading patterns with a mixed infiltrate of inflammatory cells, predominantly lymphocytes, along with plasma cells. 12 species of Candida were identified in the FEP tissues of a group of Sri Lankan male patients with oral risk habits. Among these, C. albicans was the most prevalent, with an average relative abundance of 29.56%. The other species detected included Candida tropicalis (2.59%), Candida parapsilosis (1.56%), Candida niveriensis (0.91%), Candida salmanticensis (0.74%), Candida blattae (0.13%), Candida doubliniensis (0.10%), Candida etchellsii (0.12%), Can dida metapsilosis (0.10%), Candida vanderwaltii (0.34%), Candida santamariae (0.11%), and Candida albicans_ nov_92.15% (0.002%) as shown. Candida carriage is facilitated by oral risk habits which cause chronic inflammation in the oral epithelium.
