The Effect of 15% Ocimum Basilicum L. on Lymphocyte Following Tooth Extractionin Diabetes Mellitus Wistar Rats
Abstract:
Background: Diabetes mellitus (DM) is a condition of hyperglycemia caused by abnormalities in insulin secretion, insulin action, or both. The International Diabetes Federation (IDF) notes that the prevalence of DM in the age range 75 - 79 years was calculated at 19.9% in 2019 and is anticipated to increase to 20.4% and 20.5% in 2030 and 2045. Around 75% of DM patients suffering from periodontitis and patients with DM are known to have a higher prevalence of periodontal disease than those without DM. Hyperglycemia can trigger increased production of AGEs which can inhibit the wound healing process in diabetes. Ocimum basilicum L. contains phytochemical substances, especially flavonoids, steroids, alkaloids, tannins and saponins which play a role in reducing the number of lymphocyte cells and inhibiting the production of AGEs.
Method: This study used a true experimental laboratory with a posttest only control group design. Tooth extraction was performed on rats that had been induced by alloxan and given different treatments to each group. Each group received 15% Ocimum basilicum L. extract gel, CMC-Na gel, and hyaluronic acid gel. Histopathological preparations were then observed for the number of lymphocyte cells on days 1, 3, and 5.
Results: The average number of lymphocyte cells in the group given 15% Ocimum basilicum L.
Conclusion: 15% Ocimum basilicum L. extract gel had an effect on reducing the number of lymphocyte cells on each day of observation when compared to the negative control group with the lowest mean number of lymphocyte cells in the treatment group on day 5 are 16.67 cells, this means that basil leaf extract gel 15% Ocimum basilicum L. extract gel has an effect in reducing the number of lymphocyte cells in the post-tooth extraction in inflammatory phase of wistar rats (Rattus norvegicus) with type 2 diabetes mellitus.
