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Recombinant Protein (Anti-HRP) issued from the Sea Star Igkappa Cloning by the use of CHO: Its Antibody Specificity Revealed by Elisa

Abstract:
This work was performed in the Laboratory: UMR-INRA ISP 1282 Tours (France). Dir: N.Aubrey It allows, by the use of CHO (Chinese Hamster Ovarian) protocol cloning to produce the « Young Protein » or anti-HRP (Horse-Radish Peroxydase) from the sea star IGKappa gene which corresponds to the IPA: ( Invertebrate Primitive Antibody). Two elisas confirm the anti-HRP activity.The first Elisa was done with Protein L-HRP antigen. The second one with Streptavidine-HRP at classical concentrations which are used for Mammals.

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