Abstract: Journal of Clinical and Biomedical Advances

Abstract:
In low- and middle-income nations, where treatment failures and healthcare expenses are on the rise, the growing prevalence of antimicrobial resistance (AMR) presents a combined danger to world health and economic stability. FTIR, ¹H-NMR, and ¹³C-NMR spectroscopy were used in this investigation to synthesize and characterize new phenothiazine-3-sulphonamide derivatives. Staphylococcus aureus (ATCC: 6538), Salmonella typhi (ATCC: 25175), Streptococcus pyogenes (ATCC: 27853), Escherichia coli (ATCC: 3008), and Aspergillus fumigatus (ATCC: 10231) are among the therapeutically relevant pathogens against which their antibacterial ability was evaluated using minimum inhibitory concentration (MIC) and in vitro agar well diffusion techniques. Furthermore, Auto Dock Vina's in silico molecular docking demonstrated significant binding affinities to microbial target proteins, with binding energies ranging from -5.1 to -7.6 kcal/mol. Antimicrobial activity of the synthesized compounds was on par with that of common medications like gentamycin, ketoconazole, and ciprofloxacin.