New Insights into Escherichia Coli Associated Contaminants Inplicated in Locally Fried Plantain (Dodoikire) Sold at Ile Ife and Environs, Osun State, Nigeria
Abstract:
We investigated bacterial load of thirteen samples of locally fried plantain delicacy (dodoIkire) sold randomly around IleIfe, Osun State, Nigeria and its environment. Plate count and culture methods were used for evaluation process. Sixteen bacterial isolates were obtained from the sample’s sources. Plate count revealed that the bacterial load of the samples ranged from 5.60x108CFU/ml to 4.44x109CFU/ml. The sample sold at OUI Roundabout had the highest bacterial load of 4.44x109CFU/ml while the sample bought at Lagere had the lowest bacterial load of 5.60x108CFU/ml. According to the biochemical tests result, six genera of bacteria were identified; Klebsiella spp (25%), Pseudomonas aeruginosa (25%), Yersinia spp (18.75%), E. coli (12.5%), Veillonella spp (12.5%) and Serratia spp (6.25%). Identified bacteria isolates were subjected to certain classes of antibiotics used Ciprofloxacin (5µg), Ceftazidime (30µg), Nitrofurantoin (300µg), Augmentin (30µg), Ofloxacin (5µg), Cefixime (5µg), Gentamicin (10µg), Cefuroxime (30µg). All isolates retrieved were 90% susceptible to oofloxacin (but was susceptible to the remaining antibiotics). Except Veillonella spp. that shows intermediate resistant strains factors. All isolates were resistant to augmentin antibiotic used. E. coli showed resistance to augmentin (but was susceptible to the remaining antibiotics). This finding necessitates monitoring policy and proper implementing measures, rules and regulations to be imposed on street food vendors so as to maintain standard hygienic procedures in preparation, cooking and handling of foods.
