Abstract: Journal of Clinical and Biomedical Advances

Abstract:
Laser Capture Microdissection (LCM) is an automated technique that allows the separation of cell subgroups, specific cells, or chromosomes from a heterogeneous population. Using an inverted microscope, a laser, and a sample collection system, LCM enables microscopic observation of cells, precise sectioning of regions of interest, and subsequent sample collection in a single operation. This technique is widely used in various scientific fields, particularly in cytogenetics, to isolate specific chromosomes or chromosomal regions. However, due to the small amount of DNA obtained, amplification is necessary. One of the amplification methods used was the commercial GenomePlex WGA4 Single Cell kit. In this study, chromosomes were isolated using LCM and amplified using the whole genome amplification (WGA) methodology, with the aim of using them as chromosome probes (Whole Chromosome Paints - WCP) in molecular cytogenetic analyses.