Influence of Lycopene on the mRNA Expression of Vascular Endothelial Growth Factor (VEGF) in Osteoblast Cells
Abstract:
Introduction: This research investigates the impact of lycopene, a natural carotenoid found in various fruits and vegetables, on the mRNA expression of Vascular Endothelial Growth Factor (VEGF) in MG-63 human osteoblast cells. VEGF plays a crucial role in angiogenesis, a process vital for bone formation and repair. By exploring the influence of lycopene on VEGF expression, this study aims to shed light on its potential role in enhancing bone health.
Materials and Methods: The MG-63 cells were cultured and treated with lycopene at concentrations of 1µM/ml and 10µM/ ml for 24h and 48h. Total RNA was extracted, converted to cDNA, and subjected to quantitative real-time PCR to analyze VEGF gene expression. Statistical analysis employed one-way ANOVA and Newman-Keuls tests for significance.
Results: Results revealed a significant upregulation of VEGF mRNA expression in MG-63 cells after lycopene treatment at both 24 and 48 hours. This suggests that lycopene administration may positively influence VEGF expression in osteoblast cells, potentially impacting angiogenesis and bone regeneration processes. Mean ± SD for Lycopene 1.0μM/ml is 2.15±0.49 and Lycopene 10μM/ml is 3.35±1.20 with a significance of 0.00.
Conclusion: This study demonstrates that lycopene treatment modulates VEGF mRNA expression in MG-63 human osteoblast cells, indicating its potential to promote angiogenesis and contribute to bone development and regeneration. These findings underscore the beneficial effects of lycopene on bone health, warranting further exploration of its therapeutic potential in bone-related disorders.
