Abstract: American Journal of Clinical Pathology Research

Abstract:
The present study was carried out under laboratory conditions of the Environment and National Resource Research Institute, Khartoum, Sudan, during the period April-May 2014. The objective of the study was to evaluate the effect of methanol extract of Neem (Azadeirachta indica), Fennel (Foeniculum vulgare) and Coriander (Coriandrum sativum) on the 3rd larvae of Khapra beetle (Trogoderma granarium). Three concentrations (5%, 2.5%, and 1.25%) were used for each extract. Five grams of sorghum seeds were treated separately with each extract and fed to 10 larvae in a Petri dish. The parameters measured after 29 days treatment included: mortality of the larvae, number of adult emerged and weight loss of grains. The results showed significant mortality action on the larvae. All treatments gave significant differences as compared to the control. The best result was obtained for Neem 5% extract which showed 43.8% mortality, then followed by Fennel (41.2%) and Coriander (32.2%). The control showed 7.5% mortality. The lowest mean of adult emerged after treatment of larvae was obtained by Neem 5% (31.02%), then Fennel 5% (41.9%), Coriander 5% (54.4%) and finally 81.7% for the control. There were no significant differences between the three treatments. However, there were significant differences between the treatments and control. There were no significant differences between treatments for mean weight of sorghum loss after treatment. The three treatments showed significant differences as compared to the control. The results obtained for seeds weight loss were: 2.8 %, 2%, 1.8% and 18% gm for Neem, Fennel, Coriander and control, respectively. It was concluded that application of methanol extracts of Neem, Fennel and Coriander was effective in Khapra beetle control. Concentration 5% for all extractions gave the best results. It is recommended to use 5% methanol extracts Neem and Fennel for Khapra beetle control.