Accurate Quantification of Carotenoids in Human Skin: Correcting for Melanin and Hemoglobin Interference
Abstract:|
Significance: Carotenoids are vital antioxidants in human skin, providing protection against oxidative stress and enhancing skin health. However, current non-invasive optical methods for measuring carotenoids are often limited by interference from melanin and hemoglobin, which overlap with the carotenoid absorption spectrum. Accurately quantifying carotenoids in human skin is essential for personalized health diagnostics and understanding skin health.
Aim: The goal of this study is to develop a method that corrects for melanin and hemoglobin interference, thereby improving the accuracy of non-invasive carotenoid measurements in human skin.
Approach: Using high-resolution diffuse reflection spectroscopy, we captured optical density (OD) data from a di verse population sample. Melanin correction was performed using a tangent baseline between absorbance values at 650 nm and 700 nm. Hemoglobin correction was achieved by adjusting blood OD values from 577 nm to the carotenoid absorption peak at 490 nm.
Results: This dual correction method significantly improved carotenoid measurement accuracy, yielding a more reliable assessment of carotenoid levels across different skin types and health conditions. The method reduced variability and improved consistency in carotenoid quantification.
Conclusions: The results demonstrate the importance of applying both melanin and hemoglobin corrections for accurate carotenoid quantification. This method enables more precise non-invasive assessments, supporting per sonalized health diagnostics and potential clinical applications.
