Abstract: World Journal of Molecular Medicine

Abstract:
Muscle hypertrophy relies on efficient protein synthesis, modulated by amino acid availability and signalling pathways such as mTOR, which activate muscle growth at a cellular level [1]. This study investigates the role of essential amino acids, particularly leucine and branched-chain amino acids (BCAAs), in promoting protein synthesis during resistance training. Using stable isotope labelling and molecular assays, protein synthesis rates were analysed in muscle biopsies post-intervention [2]. mTOR and associated pathways (e.g., S6K1) were assessed via Western blotting and RT-qPCR to quantify pathway activation [3]. Findings show a direct relationship between amino acid levels and increased anabolic signalling, providing data-driven insights into molecular responses essential for muscle adaptation. This research underscores targeted amino acid supplementation as a potential strategy to enhance hypertrophic outcomes in resistance training [4].