Evaluation of Healing and Proliferation Potential of Hacat Cells with the Use of the Creb Homeopathic Eye Drops
Abstract:
The popularity of homeopathic treatment for diseases has increased due to its widespread accessibility and its minimal or no side effects. Despite these advantages, the scientific evidence regarding its mechanism of action is not entirely elucidated. Thus, in vitro tests serve as primary indicators that can guarantee the initial analysis of the efficacy of medicine in general. The current study evaluated the healing and proliferation potential of HaCat cells using the CREB (Calendula, Ruta graveolens, Euphrasia, and Belladonna) homeopathic eye drops. An in vitro cell migration assay was performed to assess the tissue healing, repair, and regeneration mechanisms induced by the homeopathic eye drops containing Calendula DH3 + Ruta DH3 + Euphrasia DH3 + Belladonna DH3 (CREB). A mechanical scratch was created on the cellular layer of the human keratinocyte cell line (HaCat), and the treatment involved the application of the CREB DH3 eye drops at a concentration of 5 µL/mL. Consequently, the statistical analysis indicated that applying the CREB DH3 eye drops facilitated the closure of the scratch, exhibiting more promising outcomes than the control treatment. Therefore, this medicine emerges as a viable treatment alternative for addressing ocular disorders.
